Resuspend cells in approximately 100 l 4% formaldehyde per 1 million cells. NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. The antibodies will remain bound to the target of interest during the fixation and permeabilization process. Bookshelf Dey A,Mustafi SB,Saha S,Kumar Dhar Dwivedi S,Mukherjee P,Bhattacharya R. Chemoproteomic Evaluation of Target Engagement by the Cyclin-Dependent Kinase 4 and 6 Inhibitor Palbociclib Correlates with Cancer Cell Response. Fix for 15 min at room temperature (20-25C). Nature. P16/Ki-67 Dual Staining in Positive Human Papillomavirus DNA Testing for Predictive Diagnosis of Abnormal Cervical Lesions in Northeastern Thai Women. For reconstitution, we recommend adding 100 L distilled water to a final antibody concentration of about 1 mg/mL. Science. Pellet cells by centrifugation and remove supernatant. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. Discard supernatant in appropriate waste container. Explore pathways related to this product. BD FACSLyric Flow Cytometer Integrated with the BD FACSDuet Sample Preparation System, BD Rhapsody Express Single-Cell Analysis System, BD Rhapsody TCR/BCR Profiling Assays for Human and Mouse, BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit, Industry Solutions (Biotech, Pharma, CRO), Bulk Reagents, Custom Products and Solutions, View All Industry Solutions (Biotech, Pharma, CRO), p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l, Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development). Hallmarks of Antibody Validation, Any use of Product for diagnostic, No. addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized lines of bacterial, yeast, or insect origin are also suitable. Marx J. Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party The site you are about to visit is operated by a third party. 551011) offers all the necessary reagents to stain for this antibody. P42771. 1994; 264(5157):436-440. from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from Establishment and evaluation of the p16 INK4A FCM detection system. Show More 1/3 Image Gallery and transmitted securely. 286 citations . For Research Use Only. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Wang Z,Zhang J,Zeng Y,Sun S,Zhang J,Zhang B,Zhu M,Ouyang R,Ma B,Ye M,An X,Liu J, MicroRNA let-7g inhibited hypoxia-induced proliferation of PASMCs via G, Zhang WF,Xiong YW,Zhu TT,Xiong AZ,Bao HH,Cheng XS. NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation. Store at 4C. . All rights reserved. P16 expression can be identified by the intense brown labeling of cell nuclei (magnification 20X). Science. (Biology). Application Key: WB -Western Blot IP -Immunoprecipitation IHC -Immunohistochemistry ChIP -Chromatin Immunoprecipitation C&R -CUT&RUN C&T -CUT&Tag DB -Dot Blot eCLIP -eCLIP IF -Immunofluorescence F -Flow Cytometry Species Cross-Reactivity Key: Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis. Using this assay, we were able to quantify the activity level of SA -galactosidase, the expression level of p16 INK4a and H2AX in these cell populations. compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or Rosell-Dez A,Madisen L,Bastide S,Zeng H,Joyner AL. found p16 to be both nuclear and cytoplasmic in melanoma. Mechanisms and potential for immunotherapy. Resuspend cells in 100 l of diluted primary antibody, prepared in Antibody Dilution Buffer at a recommended dilution or as determined via titration. The p16 antibody is recommended to test for immunohistochemical staining on formalin-fixed paraffin-embedded sections. Serrano M, Hannon GJ, Beach . As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of WB: Recombinant human CDKN2A protein; CDKN2A transfected HEK293 cells; HeLa, HepG2, HEK293 cell lysate. In cohort studies, the 2-year prognostic values of p16INK4A were investigated with other risk factors by multivariate regression analyses in three cervicopathological conditions: HPV-positive Pap-normal, Pap-abnormal biopsy-negative and biopsy-confirmed LSIL. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Would you like to stay on the current country site or be switched to your country? Aliquot desired number of cells into tubes or wells. is assured; in situations where an antibody will be used to support large, long-term studies, this can be an especially Alternatively, cells may be stored overnight at 4C in 1X PBS. Zhonghua Fu Chan Ke Za Zhi. 550524) is required. Visit our. 2022 The Authors. This form is intended to help us improve our website experience. Wash by centrifugation with excess 1X PBS. Not for use in diagnostic or therapeutic procedures. The buffer is formulated with 1X phosphate-buffered saline (PBS) compatible for use with live or fixed cells, and contains bovine serum albumin (BSA) to prevent antibody aggregation. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. This antibody does not cross-react with p15 INK4B. Biochemistry (Mosc). A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Journal of assisted reproduction and genetics, Yan P,Xu J,Zeng Y,Dong G,Cao H,Zheng M,Zhu H. Knockout of 4.1B triggers malignant transformation in SV40T-immortalized mouse embryo fibroblast cells. Motterle A,Pu X,Wood H,Xiao Q,Gor S,Ng FL,Chan K,Cross F,Shohreh B,Poston RN,Tucker AT,Caulfield MJ,Ye S, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved. Not for Use in Diagnostic Procedures. Incubate for 1 hr at room temperature. Proceed to Permeabilization step. Extremely low expression of p16INK4A was observed in women with HSILs. Proceed to Permeabilization step. Protein Aliases: Clipboard, Search History, and several other advanced features are temporarily unavailable. Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified lines of bacterial, yeast, or insect origin are also suitable. Bader S,Zajac M,Friess T,Ruge E,Rieder N,Gierke B,Heubach Y,Thomas M,Pawlak M. Nrf2 status affects tumor growth, HDAC3 gene promoter associations, and the response to sulforaphane in the colon. 550878). BMC complementary and alternative medicine, Deng S,Hu B,An HM,Du Q,Xu L,Shen KP,Shi XF,Wei MM,Wu Y. Notch1 signaling contributes to the oncogenic effect of HBx on human hepatic cells. Confocal immunofluorescent analysis of HeLa cells (left, positive) or MCF7 cells (right, negative) using p16 INK4A (E6N8P) Rabbit mAb (green). Application Key: WB -Western Blot IP -Immunoprecipitation IHC -Immunohistochemistry ChIP -Chromatin Immunoprecipitation C&R -CUT&RUN C&T -CUT&Tag DB -Dot Blot eCLIP -eCLIP IF -Immunofluorescence F -Flow Cytometry Species Cross-Reactivity Key: H -Human M -Mouse UniProt ID: (Immunogen). For Research Use Only. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled Flow cytometry is a lab test used to analyze characteristics of cells or particles. BD Rhapsody Express Single-Cell Analysis System, BD Rhapsody TCR/BCR Profiling Assays for Human and Mouse, BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit, Industry Solutions (Biotech, Pharma, CRO), Bulk Reagents, Custom Products and Solutions, View All Industry Solutions (Biotech, Pharma, CRO), Intracellular staining (flow cytometry) (Routinely Tested), Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. Host server : magellan-srch-2-prod-blue:8080/10.253.226.4:8080. Store undiluted at 4C. When the p16 antibodies were no longer available from Santa Cruz, we used a different antibody (NA29, 1:100) from EMD Millipore (Billerica, MA). Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. 2023 BD. apply to Products provided by CST, its affiliates or its distributors. (Biology). in the intended application prior to experimental use. This gene generates several transcript variants which differ in their first exons. Nomanbhoy TK,Sharma G,Brown H,Wu J,Aban A,Vogeti S,Alemayehu S,Sykes M,Rosenblum JS,Kozarich JW. For research use only Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide. As p16 INK4a mRNA is readily detected in murine or human peripheral blood T cells and increases with aging . Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. Products MACS Antibodies Recombinant antibodies p16 Antibody, anti-human, REAfinity p16 Antibody, anti-human, REAfinity Clone: REA973 Type of antibody: Primary antibodies, Recombinant antibodies Isotype: recombinant human IgG1 Applications: ICFC Alternative names of antigen: p16-INK4, p16-INK4A, ARF, MTS1, CDKN2, CDK4l Choose your product Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. -, Dickson MA. Not for use in diagnostic or therapeutic procedures. Products sold or licensed by CST for antibody expression. A cell cycle regulator potentially involved in genesis of many tumor types. Key features and details Mouse monoclonal [1D7D2A1] to CDKN2A/p16INK4a Suitable for: IHC-P, WB, Flow Cyt Reacts with: Human Isotype: IgG1 You may also be interested in Primary Anti-CDKN2A/p14ARF antibody [EPR17878] (ab185620) Primary HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080) Secondary Goat Anti-Mouse IgG H&L (HRP) (ab205719) Permeabilized WI-26 VA4 cells were stained with either FITC Mouse Anti-Human p16 (Component No. 2022 Apr 27;22(1):138. doi: 10.1186/s12905-022-01714-0. While polyclonal antibodies are Lazzarini E,Balbi C,Altieri P,Pfeffer U,Gambini E,Canepa M,Varesio L,Bosco MC,Coviello D,Pompilio G,Brunelli C,Cancedda R,Ameri P,Bollini S. HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. NOTE: Count cells using a hemocytometer or alternative method. services used by Customer in connection with the Products. PMC Resuspend cells in 200-500 l of 1X PBS and analyze on flow cytometer. Background: Liu A,Guo E,Yang J,Yang Y,Liu S,Jiang X,Hu Q,Dirsch O,Dahmen U,Zhang C,Gewirtz DA,Fang H. Atorvastatin exerts inhibitory effect on endothelial senescence in hyperlipidemic rats through a mechanism involving down-regulation of miR-21-5p/203a-3p. Not for use in diagnostic procedures. No. In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic Neither the content nor the BenchSci technology and processes for Cell . NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation. Would you like to visit your country specific website? Guo Y,Li H,Ke X,Deng M,Wu Z,Cai Y,Afewerky HK,Zhang X,Pei L,Lu Y. Histone Deacetylation in the Promoter of p16 Is Involved in Fluoride-Induced Human Osteoblast Activation via the Inhibition of Sp1 Binding. Protect from light. 550880). addressing the ongoing reproducibility crisis. A comparative analysis using abnormal cervical smears with follow-up biopsies. including for use or application of the Thermo Fisher Scientific products presented. Cell cycle negative regulator beta antibody, Cyclin dependent kinase 4 inhibitor A antibody, Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody, Cyclin Dependent Kinase Inhibitor 2A antibody, Cyclin dependent kinase inhibitor 2A isoform 4 antibody, Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody, Cyclin dependent kinase inhibitor p16 antibody. Looks like you're visiting us from {{countryName}}. As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. 550946) together with the DAB Substrate Kit (Cat. Keywords: (Biology). While polyclonal antibodies are Resuspend cells in 0.5-1 ml 1X PBS. BD and the BD Logo are trademarks of Becton, Dickinson and Company. Wash cells by centrifugation in excess 1X PBS to remove methanol. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Please refer to Support Documents for Quality Certificates, Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described. Product Details Product Specific Information representative of CST, are rejected and are of no force or effect. Aqueous buffered solution containing BSA, goat serum, and 0.09% sodium azide. CST Product Terms of Sale and any applicable Unable to load your collection due to an error, Unable to load your delegates due to an error, The research profile of twotier clinical validation work for p16, The age and viral genotypedependent expression of p16, The age and lesionrelated expression patterns of p16, The ROC curves for evaluating the diagnostic performance of p16, Prospective comparison of the 2year cumulative risks of histological HSIL+ between p16. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. No. Would you like to visit your country specific website? antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. Epub 2014 May 12. cervical cancer; flow cytometry; human papillomavirus; immunostaining; p16INK4A; papanicolaou smear; pathology; quantification; squamous intraepithelial lesion. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or Mix well to dissociate pellet and prevent cross-linking of individual cells. NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water. Aliquot desired number of cells into tubes or wells. Since 2018, 24 100-women (HPV-positive/-negative, Pap-normal/-abnormal) have been enrolled nationwide for two-tier validation work. Chung SI,Moon H,Ju HL,Kim DY,Cho KJ,Ribback S,Dombrowski F,Calvisi DF,Ro SW. Testosterone Antagonizes Doxorubicin-Induced Senescence of Cardiomyocytes. (Immunogen). P16INK4A is a surrogate signature compensating for the specificity and/or sensitivity deficiencies of the human papillomavirus (HPV) DNA and Papanicolaou smear (Pap) co-test for detecting high-grade cervical squamous intraepithelial lesions or worse (HSIL+). Recombinant 130-130-708 30 tests in 60 L One test corresponds to labeling of 106 cells in a final volume of 100 L (applies only to flow cytometry). Mix well to dissociate pellet and prevent cross-linking of individual cells. No. the tissue culture supernatants of transfected host cell lines. individually using the catalog numbers listed below. BD Pharmingen Purified Mouse Anti-Human p16. Yeager T, Stadler W, Belair C, Puthenveettil J, Olopade O, Reznikoff C. Increased p16 levels correlate with pRb alterations in human urothelial cells. For Research Use Only. (Generally, 5x10. 550337) as the secondary antibody and Streptavidin-HRP (Cat. government site. Carcinogenesis associated with human papillomavirus infection. (A) Examination of, The research profile of twotier clinical validation work for p16 INK4A FCM. Xu X,Pang J,Chen Y,Bucala R,Zhang Y,Ren J. American journal of physiology. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. p16 INK4A (D7C1M) Rabbit mAb (PE Conjugate). requires a separate license from CST. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies Similarly, using antibody-based approaches, . P16INK4A FCM detected a minimal ratio of 0.01% positive cells. Wang S,Cao Z,Xue J,Li H,Jiang W,Cheng Y,Li G,Zhang X. Potency of Human Cardiosphere-Derived Cells from Patients with Ischemic Heart Disease Is Associated with Robust Vascular Supportive Ability. Methods: 550878). In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is Repeat. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. Cathepsin K knockout alleviates aging-induced cardiac dysfunction. from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells. Recombinant given assay. Yeager et al. representative of CST, are rejected and are of no force or effect. Wash by centrifugation in Antibody Dilution Buffer or 1X PBS. Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: role of autophagy. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any Analytical and performance characteristics are not established. Alternate names for p16 include p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l. After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying Chemopreventive efficacy of salvianolic acid B phospholipid complex loaded nanoparticles against experimental oral carcinogenesis: implication of sustained drug release. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. Dynamic Spatiotemporal Expression Pattern of the Senescence-Associated Factor p16Ink4a in Development and Aging. copyright notices or markings, (d) use the Products solely in accordance with For analysis by flow cytometry, peritoneal cells were washed with HBSS plus 2% heat-inactivated FBS and blocked with anti-CD16/CD32 (clone 93) for 10 min on ice . Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Comparisons are not made against non-BD technologies, unless otherwise noted. Please refer to Support Documents for Quality Certificates, Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described. -, OyervidesMuoz MA, PrezMaya AA, RodrguezGutirrez HF, et al. Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-Dependent SMAC-Mediated Apoptotic Response in Non-Small Cell Lung Cancer. No. 10883-1-AP reacts with p16 specifically. The -actin antibody was used at a 1:1000 dilution and obtained from Sigma-Aldrich (A-3853, St. Louis, MO, USA). p16 INK4A (D7C1M) Rabbit mAb (Alexa Fluor. 1993; 366(6456):704-707. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Objective: To follow the expansion of mesenchymal stem cells from umbilical cords by two classic senescence markers, p16 (INK4A) and p21 (CDKN1A), using practical, fast, and less expensive methods than the gold standard Western blotting technique, to evaluate its applicability in the laboratory. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16. 1998-2023 Abcam plc. will work as expected, to help you achieve results you can trust. waste container. Temperature: 24C, Western blot - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980), Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [1D7D2A1] (ab201980). Members of the INK4 family of cyclin dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. (Generally, 5x10. Prabakaran AD,Karakkat JV,Vijayan R,Chalissery J,Ibrahim MF,Kaimala S,Adeghate EA,Al-Marzouqi AH,Ansari SA,Mensah-Brown E,Emerald BS. 8600 Rockville Pike Visit our. Have you cited this product in a publication? Do not aliquot the antibody. Pellet cells by centrifugation and remove supernatant. They are generated by immunizing an animal with an antigen to elicit an immune response. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Cytometry Kit (Methanol) #13593, or P42771. Wang H,Zhong Q,Yang T,Qi Y,Fu M,Yang X,Qiao L,Ling Q,Liu S,Zhao Y. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Winkler C,Rouget R,Wu D,Beullens M,Van Eynde A,Bollen M. Cell-nonautonomous local and systemic responses to cell arrest enable long-bone catch-up growth in developing mice. Do not freeze. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved. The cells are then passed in front of a laser beam. Mao X,Li P,Wang Y,Liang Z,Liu J,Li J,Jiang Y,Bao G,Li L,Zhu B,Ren Y,Zhao X,Zhang J,Liu Y,Yang J,Liu P. Inhibition of BMI1 induces autophagy-mediated necroptosis. Aformalin-fixed, paraffin-embedded section from human breast tissue was stained with the Mouse Anti-Human p16 antibody (Cat. Cells were washed twice in 1 BD Perm/Wash buffer prior . For Research Use Only. Not for use in diagnostic or therapeutic procedures. found p16 to be both nuclear and cytoplasmic in melanoma. We offer antibodies conjugated to many types of fluorophores to accommodate your flow cytometry needs. Do not freeze. 2019;84(7):782799. The reviewer received a reward from Abcams Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions. Identification of High-Risk Human Papillomavirus DNA, p16, and E6/E7 Oncoproteins in Laryngeal and Hypopharyngeal Squamous Cell Carcinomas. All other trademarks are the property of their respective owners. Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. 1995; 55(3):493-497. p16 INK4A (D7C1M) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total p16 INK4A protein. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Alternatively, cells may be stored overnight at 4C in 1X PBS. Products sold or licensed by CST Any Customer's terms and conditions that are in Analyte Specific Reagent. Flow cytometric analysis was performed on a BD FACScan. Further increments were found in women with neoplastic lesions (HPV-negative: 17.7 5.0-21.4 7.2%; HPV-positive: 18.0 5.2-20.0 9.9%). copyright notices or markings, (d) use the Products solely in accordance with unlikely to become a limiting factor. Kamb A, Gruis NA, Weaver-Feldhaus J, et al. . Antibodies for Flow Cytometry. Understanding the HPV integration and its progression to cervical cancer. is assured; in situations where an antibody will be used to support large, long-term studies, this can be an especially structure or technology of the Products, or use the Products for the purpose of developing any products or services that would A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis. ACSL1 promotes imatinib-induced chronic myeloid leukemia cell senescence by regulating SIRT1/p53/p21 pathway. Chen X,Liu C,Zhao R,Zhao P,Wu J,Zhou N,Ying M. Analysis of the relationship between the KRAS G12V oncogene and the Hippo effector YAP1 in embryonal rhabdomyosarcoma. For the best web browsing experience, please use Chrome, Safari or Firefox, minimum versions 77.0.3865, 12.1.2 and 68, respectively. Moreover, since the recombinant antibody sequence is known, continuity of supply Sponsored Products Bioss Inc. CDKN2A/p16-INK4a Polyclonal Antibody Applications: WB, ELISA, FCM, ICC, IF, IHC-fr, IHC-p Reactivity: Hu, Dg Conjugate/Tag: Unconjugated Quantity: No. Thermo Fisher Scientific. antibodies and a fluorochrome labeled bacterial aerolysin (FLAER).2 Through high sensitivity flow cytometry testing, as few as 0.01% PNH cells can be detected.1 Clinical Indications This test may be useful in the evaluation of patients with intravasuclar hemolysis, unexplained hemolysis, thrombosis with unusual features, or bone marrow failure. Resuspend cells in 100 l of diluted primary antibody, prepared in Antibody Dilution Buffer at a recommended dilution or as determined via titration. Protect from light. 2020 Nov 25;55(11):784-790. doi: 10.3760/cma.j.cn112141-20200520-00428. Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Any Customer's terms and conditions that are in This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated p16 INK4A (D7C1M) Rabbit mAb #80772. Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled IHC-P: Human brain tumor, ovarian carcinoma, cervix, skin and brain tissue. FOIA Staining is nuclear and/or cytoplasmic. by the FDA or other regulatory foreign or domestic entity, for any purpose. 550880). Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. -. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. This page has been recently translated and is available in French now. Repeat if necessary. Chen Y,Pan K,Wang P,Cao Z,Wang W,Wang S,Hu N,Xue J,Li H,Jiang W,Li G,Zhang X. Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis. Journal of cellular and molecular medicine, Liu Y,Wang LL,Chen S,Zong ZH,Guan X,Zhao Y. Glutathione depletion induces ferroptosis, autophagy, and premature cell senescence in retinal pigment epithelial cells. Cancer Res. Immunohistochemistry analysis of p16 expression on human breast tissue. Gastrointestinal and liver physiology, Hamada S,Shimosegawa T,Taguchi K,Nabeshima T,Yamamoto M,Masamune A. No. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16]. Spectroscopy, Elemental and Isotope Analysis, negative regulation of cell-matrix adhesion, negative regulation of cell proliferation, negative regulation of NF-kappaB transcription factor activity, positive regulation of smooth muscle cell apoptotic process, senescence-associated heterochromatin focus assembly, negative regulation of cyclin-dependent protein serine/threonine kinase activity, negative regulation of transcription, DNA-templated, positive regulation of macrophage apoptotic process, positive regulation of cellular senescence, cyclin-dependent protein serine/threonine kinase inhibitor activity. the tissue culture supernatants of transfected host cell lines. Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated All rights reserved. 550946) together with the DAB Substrate Kit (Cat. Li Y, Nichols MA, Shay JW, Xiong Y. Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb.